I'm not sure, cause eye fatigue is setting in, but it looks like a lot of those shots are just plain missed focus, or the focus just isn't on where the objects to be examined are. (Is your AF dialed in for this lens, or how are you focusing?) (Edit: fyi, that wasn't my *eyes* so much as my browser having gotten zoomed the wrong way: they look a lot better, but I'm still suspicious of those brick wall shots and focus points.
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Even fishies don't have infinite DOF wide open. (And as someone mentioned, there's field curvature to consider. Lenses trace an arc, some a flatter one than others, you can get an idea what it is by manually-focusing and looking across the focusing screen from center to edge, where the sharpness goes. One of the virtues of my big Sigma 28 is a bit of a flatter field than most, which is nice for my style but IMO is what makes that lens also tend to totally-confound the very left-and-rightmost AF spots on my K20d.
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Certainly rather than wanting to put a ND filter on there, *stop down.
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