Originally posted by Miserere For the same subject size on the sensor, the DoF is determined ONLY by the aperture being used.
Say your subject is filling the frame lengthwise, and you're using a 50mm lens. If you use a 100mm lens and stand twice the distance away from the subject, it will still fill the frame. If you use a 200mm lens and stand 4 times as far, again, the subject will fill your frame. In all these situations the DoF will be exactly the same if you use the same aperture with every lens. That's just photography physics.
Macro at 1:1 means that a 24.5 mm long bug will fill the frame lengthwise (on an APS-C camera). Whether you're using the DA 35mm Ltd, 50mm or 100mm macro lenses doesn't matter for the DoF. If the bug fills the frame, the DoF is determined exclusively by the aperture you shoot at.
You are missing the issue Missere. For the DA 35mm ltd and the Sigma 105mm macro to get 1:1, at the same aperture, the working distance has to change because the focal length is different.
Edit: What you should be saying is that the DOF is controlled by the aperture on a given lens. Even 105mm lenses of different designs won't be exactly the same so the working distance could be slightly different.
If focal length didn't matter, I wouldn't be using a DA 35mm ltd macro and a Sigma 105mm macro. To get 1:1 and similar depth of fields from them, the working distance changes.
I don't know why you dragged the sensor back in since I agreed with you on that. the sensor size effects the angle of view or field of view. That will only change the DOF if you go back and forth with the same lens from film to 1.53x body because of the changing circle of confusion.
Edit: Edit: Did you even go to this link that has the spread sheet set up? Edit:
http://www.dofmaster.com/dofjs.html