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I have several different "main" lenses that I use between the reversed 50mm and the camera but Ill tell you about the oe I used for that shot.
The reversed 50mm 1.7 is wide open at infinity. Focusing that one wont do much difference.
For the shot I had my plastic SMC F 35-70 at 70 and I think I had it stopped at 16 but I cant be sure. That lense has a semi macro mode that can be engaged after the 70 position by that will actually zoom back out again and not get you any closer. That is when using a reversed prime infront.
Flash I use is an old Pentax AF240FT set at High Manual.
The shot was taken indoors and fairly dark. Some daylight sipping in but not much.
When I used my SMC 100 2.8 instead of the F Zoom I got alot closer. Couldnt even get the entire "fake bug" in the picture. Maybe a good combo if shooting acarids?
Another more conventional method I think was using the SMC 100 F4 Macro with extension tubes. That actually turned out pretty nice but I need new tubes. Mine are broken

Also that made the whole lense combo more then 300mm and very unpractical.
I need to get Extension tubes to use with my F Zoom. Not even sure if Auto Zoom would work with extension tubes?