Hello,
Photolady try to shoot at f/11 max f/16.
You get a better dof and don't forget : beyond f/11-f/16 the dof is better but you gain a few little more dof only.
f/11-f/16 are small apertures so need need to go up ISO top get a short time exposure.
Look at your photo, the big trouble of noise.
If you use neat image or others softs you will lose a big amount of sharpness in the process.
After f/11-f/16 you will get diffraction trouble too and a lose of sharpness again.
f/8 is really the minimum aperture too use, the dof is really thin.
Just use f/8 for short exposure time or big trouble of lightning.
The best is a handed (synchro cable or radio transmitter) flash gun with a diffuser (lumiquest ...) to use short time exposure.
Phosphene you can also try to use a TC at 1:1 macro lens shoot.
With a 2x you'll get a 2:1 magnification and with a raynox you can get more.
Of course you will lose some sharpness but with a 1.5x and dcr-150/250 with a photoshop process, the photos can be very nice.
The luminar are very expensive about 600-700 and some more than 1 000 USD.
Schneider kreuznach 5,6/135 is about 300 USD.
Meogon and Anaret are good quality enlarger photo lenses too and cheaper than Luminar.
Try using other lenses than Luminar, you get good quality and cheaper prices.
I just take a look at ebay and find very interesting lenses at very good price.
You can use nikkor microscope objectives, CFI C20 PLAN 10X is only 60 USD on ebay.
The working distance will be the trouble with these lenses.
Mikrotar by zeiss are about 300-400 USD.
If you are in trouble about working distance you can use a Micro Compound Table.
You will move the subject and not the photo gear.
Easy and more secured way to work for 100 USD.
EDIT :
here a link to a full size and a 1024 pw width preview of a ant grasping pollen on a umbelliferae.
1,5X TC + 105 mm macro + raynox DCR-150 or DCR-250 (i don't remember which raynox).
Ant size about 6 mm (about 0.236 inch).
Photo shooted handed, no tripod, no flash gun only the body flash of the K-5.
http://octopussy.fr.free.fr/pentaxforum/_IGP2649.JPG
here a example at 1024 px width (real size 1128 px width), a mayfly.
Size of the
turban-shaped
eye about 1 mm (about 10/254 inch -0.0394inch-)
Macro 1:1 and dcr-150 or dcr-250, i don't remember.