Originally posted by dappercorpmonkey Do you need to know the distance to the sensor inside the body as well there? So I take my 100mm lens^2/ the tubes +body space?^2
You are making it too complicated. Don't worry about how many stops you lose with tubes, just put your camera on X, put your flash in auto, check the flash distance for the ISO you are using, set aperture to that value (probably f8-f16) and shoot. You may need to adjust the aperture a stop or 2 either way to get what you want.
If you are not using a flash, just set the camera to manual, set the aperture to f8 to start, use the green button to meter. If the shutter speed is too low, reset the aperture until you get a higher speed.