Hi Jean,
Thank you for the welcome.
I recently setup a Nikon D7100 with the medium format Pentax 645 120mm F/4 macro lens. This is a very nice setup, since I use the smaller sensor area often to image macro subjects, with a wide range of macros that I have.
This shot was taken at ISO 400, 1/80 second hand held. I do most all my work hand held. Even the many lens that I have that are Image stabilized or Vibration Reduction controlled, I shut these features off as I do much better without that feature engaged. In numerous lab tests, I have found that there are times that these features, like others including auto-focus do not perform well enough to yield what the results that I know can be reached.
Very often I use all my cameras in the manual mode, with the lens used as a manual lens, too. This is not a problem for many lens that are adapted to the electronic cameras because there are no contacts, dependency on battery power, etc. to reduce the results that I know I can achieve by operating all the functions manually.
This shot was taken with a dark overcast present, but that is not the reason for the F/4 setting. I use most of my fine lens wide open, with little exception( like special effects or showing diffraction effects). I have lens that are faster than F/1 and others that start at F/5.6 or F/8, but little (one -two stops) or no stop down is employed. I exercise many techniques to isolate what I want to be in true focus, and do not rely upon the "false focus" introduced by stopping down the lens for more DOF. Furthermore, with few exceptions (like a poor lens, or problem lens ), stopping down a lens = compromising the performance level obtained.
Best Regards, Mike