Originally posted by FrancisK7 Dan, if I use a telephoto with a reversed small prime at the end, is the quality of one piece more important than the other?
I could use something like the 300mm f/4 with the DA21?
Then the question becomes, for that money, would I get better quality with a microscope?
The quality of both lenses is important. A DA 21mm will not work. You need a lens with an aperture ring so you can set it to wide open. There are
many inexpensive 28mm lenses from third party manufacturers that are quite high quality. Third party 24mm lenses usually cost significantly more and have more aberrations. Going below 24mm is feasible but much more costly.
As I mentioned, I know nothing about microscopy. Stone G's comments in this thread don't seem too positive. Another potential solution would be to combine two macro systems:
extension tubes +reversed short prime lens sounds promising,
extension tubes + macro lens + Raynox,
extension tubes + lens + reversed lens
Beware, you're on your way to smaller subject distances and even more miniscule DOF. Good luck, this is real specialty stuff.