With new (used) cameras in hand I'm back at it, and I've made something I'm hoping might spark more interest in this aspect of photography, so I'm adding it here.
I've found that I could quite easily make small videos of my stacks by batch resizing the intermediary images after I'm done with them. Then it's just a matter of loading the images to a stack in PS, dumping the layers to animation frames, with the final step being to export the video. Following is an example of my most ambitious stack yet, from a couple of nights ago. This is a mourning cloak butterfly's eye. The species has long been one of my favourites, so the chance to shoot them on this scale is a real win for me. I went all out, and it ended up being 1529 exposures deep. I sped this up to 60 fps for the video, so it takes less than a minute to play, but the actual shooting time was more than an hour.
Above, the finished shot, and below, a selection of others from this shoot. They're such beautiful butterflies.
This is one of the butterfly's knees, showing the joint. It's my favourite of the set, oddly enough.
These stacks range widely in depth, with a couple of them only using around 200 exposures, and a couple more around 800. They're all taken the same way with the same LED lighting.
Another fun thing that happened with the video aspect occurred to me while shooting a horse fly eye. These flies make great subjects because their eyes are huge with a kind of technicolor iridescence to them. It's the perfect time of year to collect them along roadsides and highways, as many get killed by cars. The stack looked so psychedelic while I was processing it that I dumped it to a brief video. Below that is the finished shot. It looks much nicer to me as a looping gif but I couldn't find a way to embed it in the thread, so here's the direct url.
Trippy colours in this focus stack of a horse fly's eye.
I'm pleased to entertain any questions about process. I'm no expert but I sure am enjoying the process. This is a wonderful niche of photography, the barrier for entry is lower than one would expect (my microscope was a whopping $80 CAD), and it's a great way to pass some hours at a time.