Recently I was at a butterfly conservatory, I was using my standard macro setup, DA50-200 + Raynox 250 etc... the setup works well and provides a 2.14:1 reproduction ratio at 200mm and 1.1m on the focus ring... at that magnification i'm seeing about 1.2mm in focus @ F32 .... i thought to myself how can i get more DOF without focus stacking or losing magnification... I considered how dedicated macro lenses work, nearly identical to an extension tube... you see all the elements in a dedicated macro lens move as one back and forth to obtain focus and at 1:1 magnification the lens is approx. twice as long as it would be at infinity and therefore your effective aperture is doubled, F8 dialed in would give you F16 DOF. In contrast using the Raynox F8 is still approx F8 +/- a few decimal values under the same circumstances.
Does this mean I could use extension tubes to increase my DOF while maintaining the same magnification? some said no, but i said yes! and went out and found a set of kenko extension tubes with the AE contacts... and did the testing...
Original setup...
DA50-200mm @ 200mm and F32 + Raynox 250 = 1.2mm DOF
New Setup...
DA50-200mm @ ~115mm and F29 + 68mm of tubes + Raynox 250 = 1.8mm DOF
68mm of extension tubes give me 1.5 times more DOF at the same magnification, I did have to drop the focal length to match the magnification.
Here are some examples...
Original setup (1/2 to 2/3rds of the area between the eyes are in focus)
New Setup (the edges of each eye closest to the rest of the head are in focus)
Another way to demonstrate the difference in DOF is to show how well defined the background is for a given subject. (super close-up of a Lilliput lane house)
Original setup (but at F29 instead of F32)
New setup (notice due to more DOF the background is more well defined)
Now i have a decision to make... I have a Magnicon 3X Macro Auto Tele Converter, its meant to work with 50mm F1.4 lenses and allow a maximum of 1.4:1 magnification. its actually pretty decent quality for a 3x teleconverter... However do i keep it as is or do i take it apart and remove the glass and make it a 56mm extension tube, to go with the 68mm worth of extension tubes i already have? This will allow me to have even more DOF at the same magnification.
Some photos of the macro setup
Original... (DA50-200 @ 200mm and Raynox 250)
New Setup... (Da50-200 @ ~115mm and Raynox 250 and 68mm of tubes)
The Magnicon lens and the new setup...
So i ask again... take apart the 3x converter for more DOF or leave it as is?