So there's a lot of threads right now comparing the features of camera A to camera B, but I often find that the User Interface (UI) or menu system of these cameras are often overlooked. Ergonomics is often mentioned, but I feel the UI has a lot to do with the ergonomics.
You'll often hear me say in these forums that the K-x was my first DSLR, but that's a lie, as I often forget I initially purchased a Canon T1i (500D) prior to the Pentax. Of course the T1i was sent back in favor of the K-x after playing with the Canon for about 2 weeks and the rest is history
I now own a K-7 in addition to the K-x and noticed a large step up in terms of customizing the controls.
Anyhow, I recently had a chance to play around with a friend's D90 and D3100. Aside from discussing the capability of each camera to shoot an image, I found the user interface to be awkward, albeit probably because I'm so used to the Pentax. I did find the Nikon D3100 to be very newbie friendly, as it had a picture of the aperture opening and closing when that value was changed. It also had good descriptions within the menu defining each value (shutter, ISO, aperture, etc).
Despite that, I still find Pentax seems to be more customizable in defining what functions can be assigned to the buttons or e-dials compared to the D90, T1i, or D3100.
Some of my favorite Pentax UI features off the top of my head:
zoom in or out using the rear dial for previewing shots
multi-deleting of photos by photo or folder
white balance preview
green button customization
RAW button customization
front/rear dial customization (k-7) for any mode (M, Av, Tv, Sv, etc).
Now to be fair, I should probably have given the Nikons some more time, but maybe some of you dual system shooters can chime in. What are some of your favorite UI features of Pentax? What are your favorites from other brands?