Isaac,
This is what I made of your file that you shared. I used the one that you provided with the reset white balance.
Did all the processing in PixInsight.
Steps:
Dynamic Background Extraction to eliminate some gradients
Masked Stretch
Color balance
Histogram Stretch to reset black point
Created luminance mask
Noise Reduction with mask
Masked Unsharp to bring back details on the high SNR areas
Masked Curves to drive the lower SNR areas towards black to minimize the grainy background glow
Plate solve results:
Projection origin.. [2459.416059 1629.742835]pix -> [RA:+05 35 11.59 Dec:-05 21 26.81]
Resolution ........ 2.558 arcsec/pix
Rotation .......... -58.067 deg
Focal ............. 403.19 mm
Pixel size ........ 5.00 um
Field of view ..... 3d 30' 5.5" x 2d 19' 9.1"
Image center ...... RA: 05 35 11.507 Dec: -05 21 39.81
Image bounds:
top-left ....... RA: 05 34 57.516 Dec: -03 15 46.20
top-right ...... RA: 05 27 30.589 Dec: -06 13 36.08
bottom-left .... RA: 05 42 51.118 Dec: -04 29 22.04
bottom-right ... RA: 05 35 25.596 Dec: -07 27 33.36
Here's a link to the full size jpg:
https://www.dropbox.com/s/san5fjybw8e5m57/20150923-M42Trial.jpg?dl=0 https://www.dropbox.com/s/r7ojwhkb4f89d83/20150923-M42Trial_Annotated.jpg?dl=0
Full size TIF:
https://www.dropbox.com/s/j6lar7fn76b91p7/M42Trial.tif?dl=0 https://www.dropbox.com/s/shkd5b2qlxumikz/M42Trial_Annotated.tif?dl=0
What I'd suggest doing next time:
Get to a darker site. There's still a lot of background glow. This area has a lot of dust in it and this is only visible with longer exposures and darker skies.
Shoot when your target is high in the sky, at least 40 degrees above the horizon. This will minimize the amount of atmospheric distortion and filtering of the light.
Lower your ISO setting. Many of the stars are clipped and thus the true color is lost.
More subs. Lots more. With such short durations, I'd suggest at least 64.